Publication:
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation.

dc.contributor.authorMoura, David S
dc.contributor.authorFernandez, Isabel F
dc.contributor.authorMarin-Royo, Gema
dc.contributor.authorLopez-Sanchez, Inmaculada
dc.contributor.authorMartin-Doncel, Elena
dc.contributor.authorVega, Francisco M
dc.contributor.authorLazo, Pedro A
dc.contributor.funderMinisterio de Economía y Competitividad
dc.contributor.funderConsejería de Educación de la Junta de Castilla y León
dc.date.accessioned2023-01-25T08:33:39Z
dc.date.available2023-01-25T08:33:39Z
dc.date.issued2016-06-23
dc.description.abstractSox2 is a pluripotency transcription factor that as an oncogene can also regulate cell proliferation. Therefore, genes implicated in several different aspects of cell proliferation, such as the VRK1 chromatin-kinase, are candidates to be targets of Sox2. Sox 2 and VRK1 colocalize in nuclei of proliferating cells forming a stable complex. Sox2 knockdown abrogates VRK1 gene expression. Depletion of either Sox2 or VRK1 caused a reduction of cell proliferation. Sox2 up-regulates VRK1 expression and both proteins cooperate in the activation of CCND1. The accumulation of VRK1 protein downregulates SOX2 expression and both proteins are lost in terminally differentiated cells. Induction of neural differentiation with retinoic acid resulted in downregulation of Sox2 and VRK1 that inversely correlated with the expression of differentiation markers such as N-cadherin, Pax6, mH2A1.2 and mH2A2. Differentiation-associated macro histones mH2A1.2and mH2A2 inhibit CCND1 and VRK1 expression and also block the activation of the VRK1 promoter by Sox2. VRK1 is a downstream target of Sox2 and both form an autoregulatory loop in epithelial cell differentiation.
dc.description.sponsorshipDSM and IL-S were supported by MINECO-FPI predoctoral fellowships. IFF was supported by JAE-CSIC-Fondo Social Europeo predoctoral fellowship. EM-D was supported by JCyL-Consejería de Educación-Fondo Social Europeo predoctoral fellowship. This work was funded by grants from Ministerio de Economía y Competitividad (SAF2013-44810R, SAF2014-57791-REDC) and Consejería de Educación de la Junta de Castilla y León (CSI002U14 and UIC-017) to P.A.L.
dc.description.versionSi
dc.identifier.citationMoura DS, Fernández IF, Marín-Royo G, López-Sánchez I, Martín-Doncel E, Vega FM,et al. Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation. Sci Rep. 2016 Jun 23;6:28532.
dc.identifier.doi10.1038/srep28532
dc.identifier.essn2045-2322
dc.identifier.pmcPMC4917848
dc.identifier.pmid27334688
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917848/pdf
dc.identifier.unpaywallURLhttps://www.nature.com/articles/srep28532.pdf
dc.identifier.urihttp://hdl.handle.net/10668/10207
dc.journal.titleScientific reports
dc.journal.titleabbreviationSci Rep
dc.language.isoen
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.organizationHospital Universitario Virgen del Rocío
dc.page.number15
dc.provenanceRealizada la curación de contenido 23/06/2025.
dc.publisherNature Publishing Group
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.relation.projectIDSAF2013-44810R
dc.relation.projectIDSAF2014-57791-REDC
dc.relation.projectIDCSI002U14
dc.relation.projectIDUIC-017
dc.relation.publisherversionhttps://doi.org/10.1038/srep28532
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSox2
dc.subjectVrk1
dc.subjectCell proliferation
dc.subjectTranscription factor
dc.subjectCellular differentiation
dc.subjectCyclin D1
dc.subject.decsSox2
dc.subject.decsVrk1
dc.subject.decsProliferación celular
dc.subject.decsFactor de transcripción
dc.subject.decsDiferenciación celular
dc.subject.decsCiclin D1
dc.subject.meshBiomarkers
dc.subject.meshCadherins
dc.subject.meshCarcinogenesis
dc.subject.meshCell Cycle
dc.subject.meshCell Differentiation
dc.subject.meshCell Line
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Proliferation
dc.subject.meshCyclin D1
dc.subject.meshDown-Regulation
dc.subject.meshEpithelium
dc.subject.meshHEK293 Cells
dc.subject.meshHeLa Cells
dc.subject.meshHumans
dc.subject.meshIntracellular Signaling Peptides and Proteins
dc.subject.meshMCF-7 Cells
dc.subject.meshOncogenes
dc.subject.meshPAX6 Transcription Factor
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshProtein Serine-Threonine Kinases
dc.subject.meshSOXB1 Transcription Factors
dc.subject.meshUp-Regulation
dc.titleOncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number6
dspace.entity.typePublication

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