Publication: Complementary Functions of Plant AP Endonucleases and AP Lyases during DNA Repair of Abasic Sites Arising from C:G Base Pairs
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Identifiers
Date
2021-08-16
Authors
Jordano-Raya, Marina
Beltrán-Melero, Cristina
Moreno-Recio, M. Dolores
Martínez-Macías, M. Isabel
Ariza, Rafael R.
Roldán-Arjona, Teresa
Córdoba-Cañero, Dolores
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Abasic (apurinic/apyrimidinic, AP) sites are ubiquitous DNA lesions arising from spontaneous base loss and excision of damaged bases. They may be processed either by AP endonucleases or AP lyases, but the relative roles of these two classes of enzymes are not well understood. We hypothesized that endonucleases and lyases may be differentially influenced by the sequence surrounding the AP site and/or the identity of the orphan base. To test this idea, we analysed the activity of plant and human AP endonucleases and AP lyases on DNA substrates containing an abasic site opposite either G or C in different sequence contexts. AP sites opposite G are common intermediates during the repair of deaminated cytosines, whereas AP sites opposite C frequently arise from oxidized guanines. We found that the major Arabidopsis AP endonuclease (ARP) exhibited a higher efficiency on AP sites opposite G. In contrast, the main plant AP lyase (FPG) showed a greater preference for AP sites opposite C. The major human AP endonuclease (APE1) preferred G as the orphan base, but only in some sequence contexts. We propose that plant AP endonucleases and AP lyases play complementary DNA repair functions on abasic sites arising at C:G pairs, neutralizing the potential mutagenic consequences of C deamination and G oxidation, respectively.
Description
MeSH Terms
Medical Subject Headings::Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Brassicaceae::Arabidopsis
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Binding Sites
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::DNA Repair Enzymes::DNA-(Apurinic or Apyrimidinic Site) Lyase
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Endonucleases
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Substrate Specificity
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Nucleic Acid Hybridization::Base Pairing
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Damage
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::DNA Repair
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Single-Stranded::DNA, Complementary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Deamination
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Binding Sites
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::DNA Repair Enzymes::DNA-(Apurinic or Apyrimidinic Site) Lyase
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Endonucleases
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Substrate Specificity
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Nucleic Acid Hybridization::Base Pairing
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Damage
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::DNA Repair
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Single-Stranded::DNA, Complementary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Deamination
DeCS Terms
CIE Terms
Keywords
Abasic sites, AP endonucleases, AP lyases, Base excision repair, Arabidopsis, Deamination, Endonucleasas, Liasas, Reparación del ADN, Desaminación, Daño del ADN
Citation
Jordano-Raya M, Beltrán-Melero C, Moreno-Recio MD, Martínez-Macías MI, Ariza RR, Roldán-Arjona T, et al. Complementary Functions of Plant AP Endonucleases and AP Lyases during DNA Repair of Abasic Sites Arising from C:G Base Pairs. Int J Mol Sci. 2021 Aug 16;22(16):8763.