Publication:
Measurement of Serum Testosterone in Nondiabetic Young Obese Men: Comparison of Direct Immunoassay to Liquid Chromatography-Tandem Mass Spectrometry.

dc.contributor.authorMartínez-Escribano, Ana
dc.contributor.authorMaroto-García, Julia
dc.contributor.authorRuiz-Galdón, Maximiliano
dc.contributor.authorBarrios-Rodríguez, Rocío
dc.contributor.authorÁlvarez-Millán, Juan J
dc.contributor.authorCabezas-Sánchez, Pablo
dc.contributor.authorPlaza-Andrades, Isaac
dc.contributor.authorMolina-Vega, María
dc.contributor.authorTinahones, Francisco J
dc.contributor.authorQueipo-Ortuño, María Isabel
dc.contributor.authorFernández-García, José Carlos
dc.date.accessioned2023-02-09T10:38:35Z
dc.date.available2023-02-09T10:38:35Z
dc.date.issued2020-12-19
dc.description.abstractHypoandrogenemia, a frequent finding in men with obesity, is defined by low concentrations of serum testosterone. Although immunoassay (IA) is the most used method for the determination of this steroid in clinical practice, liquid chromatography-mass spectrometry (LC-MS/MS) is considered a more reliable method. In this study, we aimed to compare IA versus LC-MS/MS measurement for the diagnosis of hypoandrogenemia in a cohort of 273 nondiabetic young obese men. Mean total testosterone (TT) levels were 3.20 ± 1.24 ng/mL for IA and 3.78 ± 1.4 ng/mL for LC-MS/MS. 53.7% and 26.3% of patients were classified as presenting hypoandrogenemia with IA and LC-MS/MS, respectively. Considering LC-MS/MS as the reference method, sensitivity and specificity of IA were 91.4% (95% CI 82.3-96.8) and 61.1% (95% CI 54.0-67.8), respectively. IA presented an AUC of 0.879 (95% CI 0.83-0.928). Multivariate regression analysis indicated that sex hormone-binding globulin (SHBG) concentrations (p = 0.002) and insulin resistance (p = 0.008) were factors associated with discrepant IA values. In conclusion, the determination of TT by IA in nondiabetic young men with obesity yields lower concentrations of TT than LC-MS/MS, resulting in an equivocal increased diagnosis of hypoandrogenemia, which could lead to inaccurate diagnosis and unnecessary treatment.
dc.identifier.doi10.3390/biom10121697
dc.identifier.essn2218-273X
dc.identifier.pmcPMC7765982
dc.identifier.pmid33352636
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765982/pdf
dc.identifier.unpaywallURLhttps://www.mdpi.com/2218-273X/10/12/1697/pdf?version=1608600186
dc.identifier.urihttp://hdl.handle.net/10668/16834
dc.issue.number12
dc.journal.titleBiomolecules
dc.journal.titleabbreviationBiomolecules
dc.language.isoen
dc.organizationIBIMA
dc.organizationIBS
dc.pubmedtypeComparative Study
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectchromatography
dc.subjectimmunoassay
dc.subjectobesity
dc.subjecttestosterone
dc.subject.meshAdult
dc.subject.meshChromatography
dc.subject.meshChromatography, Liquid
dc.subject.meshHumans
dc.subject.meshImmunoassay
dc.subject.meshLimit of Detection
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshObesity
dc.subject.meshRegression Analysis
dc.subject.meshReproducibility of Results
dc.subject.meshSensitivity and Specificity
dc.subject.meshTandem Mass Spectrometry
dc.subject.meshTestosterone
dc.titleMeasurement of Serum Testosterone in Nondiabetic Young Obese Men: Comparison of Direct Immunoassay to Liquid Chromatography-Tandem Mass Spectrometry.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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