Publication: Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
dc.contributor.author | Gil-Ocaña, Violeta | |
dc.contributor.author | Jimenez, Isabel M. | |
dc.contributor.author | Mayorga, Cristobalina | |
dc.contributor.author | Doña, Inmaculada | |
dc.contributor.author | Céspedes, Jose Antonio | |
dc.contributor.author | Montañez, Maria I. | |
dc.contributor.author | Vida, Yolanda | |
dc.contributor.author | Torres, Maria J. | |
dc.contributor.author | Perez-Inestrosa, Ezequiel | |
dc.contributor.authoraffiliation | [Gil-Ocaña,V; Vida,Y; Perez-Inestrosa,E] Universidad de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Dpto. Química Orgánica, Málaga, Spain. [Gil-Ocaña,V; Mayorga,C; Montañez,MI; Vida,Y; Torres,MJ; Perez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología-BIONAND, Parque Tecnológico de Andalucía, Málaga, Spain. [Jimenez,IM; Mayorga,C; Doña,I; Céspedes,JA.; Montañez,MI; Vida,Y; Torres,MJ] Allergy Research Group, Instituto de Investigación Biomédica de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain. [Jimenez,IM; Mayorga,C; Doña,I; Montañez,MI; Vida,Y; Torres,MJ] Allergy Unit, Hospital Regional Universitario de Málaga, Málaga, Spain. [Torres,MJ] Universidad de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Dpto. Medicina, Málaga, Spain. | |
dc.contributor.funder | This work was supported by the Spanish Ministerio de Ciencia e Innovación (Proyectos de I+D+I «Programación Conjunta Internacional», EuroNanoMed 2019 (PCI2019-111825-2), Ministerio de Ciencia y Educación (PID2019-104293GB-I00), Instituto de Salud Carlos III (ISCIII) of MINECO (grants cofunded by ERDF: ‘‘Una manera de hacer Europa’’ (Euronanomed Program AC19/00082, PI20/01734, PI18/00095, RETIC ARADYAL, RD16/0006/0001 and RD16/0006/0012, and Miguel Servet II program (CPII20/00028)), Junta de Andalucía and Universidad de Málaga (UMA18-FEDERJA-007), Andalusian Regional Ministry of Health (PE-0172-2018) and Nicolas Monardes Program (RC-0004-2021). | |
dc.date.accessioned | 2022-03-02T11:09:12Z | |
dc.date.available | 2022-03-02T11:09:12Z | |
dc.date.issued | 2021-12-03 | |
dc.description.abstract | β-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug-protein conjugates that both induce the reactions and are immunologically recognized. Dendrimeric Antigen-Silica particle composites (DeAn@SiO2), consisting on nanoparticles decorated with BL-DeAns are promising candidates for improving the in vitro clinical diagnostic practice. In this nano-inspired system biology, the synthetic dendrimer plays the role of the natural carrier protein, emulating its haptenation by drugs and amplifying the multivalence. Herein, we present the design and synthesis of new multivalent mono- and bi-epitope DeAn@SiO2, using amoxicillin and/or benzylpenicillin allergenic determinants as ligands. The homogeneous composition of nanoparticles provides high reproducibility and quality, which is critical for in vitro applications. The suitable functionalization of nanoparticles allows the anchoring of DeAn, minimizing the nonspecific interactions and facilitating the effective exposure to specific IgE; while the larger interaction area increments the likelihood of capturing specific IgE. This achievement is particularly important for improving sensitivity of current immunoassays since IgE levels in BL allergic patients are very low. Our data suggest that these new nano-based platforms provide a suitable tool for testing IgE recognition to more than one BL simultaneously. Immunochemical studies evidence that mono and bi-epitope DeAn@SiO2 composites could potentially allow the diagnosis of patients allergic to any of these drugs with a single test. These organic-inorganic hybrid materials represent the basis for the development of a single screening for BL-allergies. | es_ES |
dc.description.version | Yes | es_ES |
dc.identifier.citation | Gil-Ocaña V, Jimenez IM, Mayorga C, Doña I, Céspedes JA, Montañez MI, et al. Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs. Front Immunol. 2021 Dec 3;12:750109. | es_ES |
dc.identifier.doi | 10.3389/fimmu.2021.750109 | es_ES |
dc.identifier.essn | 1664-3224 | |
dc.identifier.pmc | PMC8678411 | |
dc.identifier.pmid | 34925325 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10668/3456 | |
dc.journal.title | Frontiers in Immunology | |
dc.language.iso | en | |
dc.page.number | 13 p. | |
dc.publisher | Frontiers | es_ES |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fimmu.2021.750109/full | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Dendrimeric antigen | es_ES |
dc.subject | Silica nanoparticles | es_ES |
dc.subject | Biepitope nanocomposites | es_ES |
dc.subject | Diagnostic test | es_ES |
dc.subject | Drug allergy | es_ES |
dc.subject | Penicillins | es_ES |
dc.subject | Specific IgE | es_ES |
dc.subject | Aprobación de pruebas de diagnóstico | es_ES |
dc.subject | Hipersensibilidad a las drogas | es_ES |
dc.subject | Penicilinas | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adolescent | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Aged | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Biological Factors:: | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Dendrimers | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Reproductive Control Agents::Fertility Agents::Fertility Agents, Female | es_ES |
dc.subject.mesh | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Men::Nurses, Male | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Middle Aged | es_ES |
dc.subject.mesh | Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Silicon Compounds::Silicon Dioxide | es_ES |
dc.subject.mesh | Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Minerals::Silicon Dioxide | es_ES |
dc.subject.mesh | Medical Subject Headings::Persons::Persons::Age Groups::Adult::Young Adult | es_ES |
dc.title | Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs | es_ES |
dc.type | research article | |
dc.type.hasVersion | VoR | |
dspace.entity.type | Publication |
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