Publication:
Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs

dc.contributor.authorGil-Ocaña, Violeta
dc.contributor.authorJimenez, Isabel M.
dc.contributor.authorMayorga, Cristobalina
dc.contributor.authorDoña, Inmaculada
dc.contributor.authorCéspedes, Jose Antonio
dc.contributor.authorMontañez, Maria I.
dc.contributor.authorVida, Yolanda
dc.contributor.authorTorres, Maria J.
dc.contributor.authorPerez-Inestrosa, Ezequiel
dc.contributor.authoraffiliation[Gil-Ocaña,V; Vida,Y; Perez-Inestrosa,E] Universidad de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Dpto. Química Orgánica, Málaga, Spain. [Gil-Ocaña,V; Mayorga,C; Montañez,MI; Vida,Y; Torres,MJ; Perez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología-BIONAND, Parque Tecnológico de Andalucía, Málaga, Spain. [Jimenez,IM; Mayorga,C; Doña,I; Céspedes,JA.; Montañez,MI; Vida,Y; Torres,MJ] Allergy Research Group, Instituto de Investigación Biomédica de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain. [Jimenez,IM; Mayorga,C; Doña,I; Montañez,MI; Vida,Y; Torres,MJ] Allergy Unit, Hospital Regional Universitario de Málaga, Málaga, Spain. [Torres,MJ] Universidad de Málaga-Instituto de Investigación Biomédica de Málaga (IBIMA), Dpto. Medicina, Málaga, Spain.
dc.contributor.funderThis work was supported by the Spanish Ministerio de Ciencia e Innovación (Proyectos de I+D+I «Programación Conjunta Internacional», EuroNanoMed 2019 (PCI2019-111825-2), Ministerio de Ciencia y Educación (PID2019-104293GB-I00), Instituto de Salud Carlos III (ISCIII) of MINECO (grants cofunded by ERDF: ‘‘Una manera de hacer Europa’’ (Euronanomed Program AC19/00082, PI20/01734, PI18/00095, RETIC ARADYAL, RD16/0006/0001 and RD16/0006/0012, and Miguel Servet II program (CPII20/00028)), Junta de Andalucía and Universidad de Málaga (UMA18-FEDERJA-007), Andalusian Regional Ministry of Health (PE-0172-2018) and Nicolas Monardes Program (RC-0004-2021).
dc.date.accessioned2022-03-02T11:09:12Z
dc.date.available2022-03-02T11:09:12Z
dc.date.issued2021-12-03
dc.description.abstractβ-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug-protein conjugates that both induce the reactions and are immunologically recognized. Dendrimeric Antigen-Silica particle composites (DeAn@SiO2), consisting on nanoparticles decorated with BL-DeAns are promising candidates for improving the in vitro clinical diagnostic practice. In this nano-inspired system biology, the synthetic dendrimer plays the role of the natural carrier protein, emulating its haptenation by drugs and amplifying the multivalence. Herein, we present the design and synthesis of new multivalent mono- and bi-epitope DeAn@SiO2, using amoxicillin and/or benzylpenicillin allergenic determinants as ligands. The homogeneous composition of nanoparticles provides high reproducibility and quality, which is critical for in vitro applications. The suitable functionalization of nanoparticles allows the anchoring of DeAn, minimizing the nonspecific interactions and facilitating the effective exposure to specific IgE; while the larger interaction area increments the likelihood of capturing specific IgE. This achievement is particularly important for improving sensitivity of current immunoassays since IgE levels in BL allergic patients are very low. Our data suggest that these new nano-based platforms provide a suitable tool for testing IgE recognition to more than one BL simultaneously. Immunochemical studies evidence that mono and bi-epitope DeAn@SiO2 composites could potentially allow the diagnosis of patients allergic to any of these drugs with a single test. These organic-inorganic hybrid materials represent the basis for the development of a single screening for BL-allergies.es_ES
dc.description.versionYeses_ES
dc.identifier.citationGil-Ocaña V, Jimenez IM, Mayorga C, Doña I, Céspedes JA, Montañez MI, et al. Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs. Front Immunol. 2021 Dec 3;12:750109.es_ES
dc.identifier.doi10.3389/fimmu.2021.750109es_ES
dc.identifier.essn1664-3224
dc.identifier.pmcPMC8678411
dc.identifier.pmid34925325es_ES
dc.identifier.urihttp://hdl.handle.net/10668/3456
dc.journal.titleFrontiers in Immunology
dc.language.isoen
dc.page.number13 p.
dc.publisherFrontierses_ES
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.750109/fulles_ES
dc.rightsAtribución 4.0 Internacional*
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDendrimeric antigenes_ES
dc.subjectSilica nanoparticleses_ES
dc.subjectBiepitope nanocompositeses_ES
dc.subjectDiagnostic testes_ES
dc.subjectDrug allergyes_ES
dc.subjectPenicillinses_ES
dc.subjectSpecific IgEes_ES
dc.subjectAprobación de pruebas de diagnósticoes_ES
dc.subjectHipersensibilidad a las drogases_ES
dc.subjectPenicilinases_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adolescentes_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adultes_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Agedes_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::es_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigenses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Dendrimerses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Reproductive Control Agents::Fertility Agents::Fertility Agents, Femalees_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin Ees_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Men::Nurses, Malees_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Middle Agedes_ES
dc.subject.meshMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Nanostructures::Nanoparticleses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Silicon Compounds::Silicon Dioxidees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Minerals::Silicon Dioxidees_ES
dc.subject.meshMedical Subject Headings::Persons::Persons::Age Groups::Adult::Young Adultes_ES
dc.titleMultiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEses_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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