Publication: Validation of the 1,4-butanediol thermoplastic polyurethane as a novel material for 3D bioprinting applications
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Identifiers
Date
2020-10-20
Authors
Chocarro-Wrona, Carlos
de Vicente, Juan
Antich, Cristina
Jiménez, Gema
Martínez-Moreno, Daniel
Carrillo, Esmeralda
Montañez, Elvira
Gálvez-Martín, Patricia
Perán, Macarena
López-Ruiz, Elena
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley Periodicals LLC, on behalf of American Institute of Chemical Engineers
Abstract
Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires the development of functional personalized implants with cartilage-like mechanical properties capable of sustaining high load-bearing environments to integrate into the surrounding tissue of the cartilage defect. In this study, we evaluated the novel 1,4-butanediol thermoplastic polyurethane elastomer (b-TPUe) derivative filament as a 3D bioprinting material with application in cartilage TE. The mechanical behavior of b-TPUe in terms of friction and elasticity were examined and compared with human articular cartilage, PCL, and PLA. Moreover, infrapatellar fat pad-derived human mesenchymal stem cells (MSCs) were bioprinted together with scaffolds. in vitro cytotoxicity, proliferative potential, cell viability, and chondrogenic differentiation were analyzed by Alamar blue assay, SEM, confocal microscopy, and RT-qPCR. Moreover, in vivo biocompatibility and host integration were analyzed. b-TPUe demonstrated a much closer compression and shear behavior to native cartilage than PCL and PLA, as well as closer tribological properties to cartilage. Moreover, b-TPUe bioprinted scaffolds were able to maintain proper proliferative potential, cell viability, and supported MSCs chondrogenesis. Finally, in vivo studies revealed no toxic effects 21 days after scaffolds implantation, extracellular matrix deposition and integration within the surrounding tissue. This is the first study that validates the biocompatibility of b-TPUe for 3D bioprinting. Our findings indicate that this biomaterial can be exploited for the automated biofabrication of artificial tissues with tailorable mechanical properties including the great potential for cartilage TE applications.
Description
MeSH Terms
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Chondrogenesis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Bioprinting
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Elastomers::Polyurethanes
Medical Subject Headings::Anatomy::Musculoskeletal System::Cartilage::Hyaline Cartilage::Cartilage, Articular
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Friction
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Mechanical Processes::Weight-Bearing
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Biocompatible Materials
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Polyesters
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Scleroproteins::Extracellular Matrix Proteins
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Confocal
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Alcohols::Glycols::Butylene Glycols
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Elasticity
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Chondrogenesis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Bioprinting
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Elastomers::Polyurethanes
Medical Subject Headings::Anatomy::Musculoskeletal System::Cartilage::Hyaline Cartilage::Cartilage, Articular
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Friction
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Mechanical Processes::Weight-Bearing
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Biocompatible Materials
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Polyesters
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Scleroproteins::Extracellular Matrix Proteins
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Confocal
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Alcohols::Glycols::Butylene Glycols
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Elasticity
DeCS Terms
CIE Terms
Keywords
1,4-butanediol thermoplastic polyurethane, 3D bioprinting, MSCs, Elastomer, Tissue engineering, Chondrogenesis, Poliuretanos, Bioimpresión, Elastómeros, Ingeniería de tejidos, Condrogénesis, Células madre mesenquimatosas
Citation
Chocarro-Wrona C, de Vicente J, Antich C, Jiménez G, Martínez-Moreno D, Carrillo E, et al. Validation of the 1,4-butanediol thermoplastic polyurethane as a novel material for 3D bioprinting applications. Bioeng Transl Med. 2020 Nov 14;6(1):e10192.