Publication: Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs.
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Identifiers
Date
2016-05
Authors
Fontán-Lozano, A
Capilla-Gonzalez, V
Aguilera, Y
Mellado, N
Carrión, A M
Soria, B
Hmadcha, A
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
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Abstract
Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are involved in maintaining ESC self-renewal and pluripotency. However, a previous knockout study revealed the protective function of DREAM depletion in brain aging degeneration and that aging is accompanied by a progressive decline in stem cells (SCs) function. Interestingly, we found that DREAM is expressed in different cell types, including human ESCs (hESCs), human adipose-derived stromal cells (hASCs), human bone marrow-derived stromal cells (hBMSCs), and human newborn foreskin fibroblasts (hFFs), and that transitory inhibition of DREAM in hESCs reduces their pluripotency, increasing differentiation. We stipulate that these changes are partly mediated by increased CREB transcriptional activity. Overall, our data indicates that DREAM acts in the regulation of hESC pluripotency and could be a target to promote or prevent differentiation in embryonic cells.
Description
Journal Article;
MeSH Terms
Medical Subject Headings::Anatomy::Cells::Stem Cells::Embryonic Stem Cells::Human Embryonic Stem Cells
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Calcium-Binding Proteins::Intracellular Calcium-Sensing Proteins::Neuronal Calcium-Sensor Proteins::Kv Channel-Interacting Proteins
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Cyclic AMP Response Element-Binding Protein
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Acetyltransferases::p300-CBP Transcription Factors::CREB-Binding Protein
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Fibroblasts
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Calcium-Binding Proteins::Intracellular Calcium-Sensing Proteins::Neuronal Calcium-Sensor Proteins::Kv Channel-Interacting Proteins
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Cyclic AMP Response Element-Binding Protein
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Acetyltransferases::p300-CBP Transcription Factors::CREB-Binding Protein
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Fibroblasts
DeCS Terms
CIE Terms
Keywords
Human embryonic stem cells, Pluripotency, Differentiation, DREAM, KChIP-3, Calsenilin, CREB, Células madre embrionarias, Diferenciación celular
Citation
Fontán-Lozano A, Capilla-Gonzalez V, Aguilera Y, Mellado N, Carrión AM, Soria B, et al. Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs. Stem Cell Res. 2016; 16(3):568-78