Publication:
Inflammatory response in human lung cells stimulated with plasma from COPD patients.

dc.contributor.authorArellano-Orden, Elena
dc.contributor.authorCalero-Acuña, Carmen
dc.contributor.authorSánchez-López, Verónica
dc.contributor.authorCarrasco-Hernández, Laura
dc.contributor.authorMárquez-Martín, Eduardo
dc.contributor.authorOrtega-Ruiz, Francisco
dc.contributor.authorOtero-Candelera, Remedios
dc.contributor.authorMarín-Hinojosa, Carmen
dc.contributor.authorLópez-Campos, José Luis
dc.date.accessioned2023-05-03T14:24:59Z
dc.date.available2023-05-03T14:24:59Z
dc.date.issued2022-05-24
dc.description.abstractChronic obstructive pulmonary disease (COPD) is a condition resulting from a persistent inflammatory state in the airways even after smoking cessation. Intriguingly, the reasons behind this persistence of the inflammatory influx without smoking exposure have not been fully unraveled. We aimed to explore the hypothesis that systemic inflammation in COPD patients influences lung cell inflammatory response. We cultured human lung fibroblast and human airway epithelial cell lines with plasma from COPD patients (four emphysematous-COPD, four asthma-COPD overlap, four chronic bronchitis-COPD, and four bronchiectasis- COPD), and four smokers or ex-smokers without COPD as controls. Non-stimulated cells were used as controls. We measured Interleukine-8 (IL-8), C-reactive protein (CRP) and matrix metalloproteinase-9 (MMP-9) in plasma and culture supernatants by ELISA. Cells stimulated with plasma from COPD patients and non-COPD smoker subjects produced higher CRP, IL- 8 and MMP-9 levels, an increase for COPD in CRP (p=0.029) in epithelial cells and IL-8 (p=0.039) in fibroblasts and decrease for MMP-9 (p=0.039) in fibroblasts, compared with non-stimulated cells. The response was higher in epithelial cells for IL-8 (p=0.003) and in fibroblasts for MMP-9 (p=0.063). The plasma from chronic bronchitis and bronchiectasis phenotypes induced higher IL-8 in fibroblasts. Plasma from COPD patients increases the inflammatory response in lung epithelial cells and lung fibroblasts, with a different response depending on the cell type and clinical phenotype.
dc.identifier.doi10.4081/mrm.2022.817
dc.identifier.issn1828-695X
dc.identifier.pmcPMC9179873
dc.identifier.pmid35692377
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179873/pdf
dc.identifier.unpaywallURLhttps://mrmjournal.org/mrm/article/download/817/1350
dc.identifier.urihttp://hdl.handle.net/10668/21621
dc.journal.titleMultidisciplinary respiratory medicine
dc.journal.titleabbreviationMultidiscip Respir Med
dc.language.isoen
dc.organizationHospital Universitario Virgen del Rocío
dc.organizationHospital Universitario Virgen del Rocío
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.page.number817
dc.pubmedtypeJournal Article
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCOPD
dc.subjectCRP
dc.subjectIL-8
dc.subjectMMP-9
dc.subjectinflammation
dc.titleInflammatory response in human lung cells stimulated with plasma from COPD patients.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number17
dspace.entity.typePublication

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