Publication:
CtIP -mediated alternative mRNA splicing finetunes the DNA damage response.

dc.contributor.authorPrados-Carvajal, Rosario
dc.contributor.authorRodriguez-Real, Guillermo
dc.contributor.authorGutierrez-Pozo, Gabriel
dc.contributor.authorHuertas, Pablo
dc.date.accessioned2023-02-09T10:38:11Z
dc.date.available2023-02-09T10:38:11Z
dc.date.issued2020-12-09
dc.description.abstractIn order to survive to the exposure of DNA damaging agents, cells activate a complex response that coordinates the cellular metabolism, cell cycle progression and DNA repair. Among many other events, recent evidence has described global changes in mRNA splicing in cells treated with genotoxic agents. Here, we explore further this DNA damage-dependent alternative splicing. Indeed, we show that both the splicing factor SF3B2 and the repair protein CtIP contribute to the global pattern of splicing both in cells treated or not to DNA damaging agents. Additionally, we focus on a specific DNA damage- and CtIP-dependent alternative splicing event of the helicase PIF1 and explore its relevance for the survival of cells upon exposure to ionizing radiation. Indeed, we described how the nuclear, active form of PIF1 is substituted by a splicing variant, named vPIF1, in a fashion that requires both the presence of DNA damage and CtIP. Interestingly, timely expression of vPIF1 is required for optimal survival to exposure to DNA damaging agents, but early expression of this isoform delays early events of the DNA damage response. On the contrary, expression of the full length PIF1 facilitates those early events, but increases the sensitivity to DNA damaging agents if the expression is maintained long-term.
dc.identifier.doi10.1261/rna.078519.120
dc.identifier.essn1469-9001
dc.identifier.pmcPMC7901839
dc.identifier.pmid33298529
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901839/pdf
dc.identifier.unpaywallURLhttp://rnajournal.cshlp.org/content/27/3/303.full.pdf
dc.identifier.urihttp://hdl.handle.net/10668/16765
dc.issue.number3
dc.journal.titleRNA (New York, N.Y.)
dc.journal.titleabbreviationRNA
dc.language.isoen
dc.organizationCentro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER
dc.page.number303-23
dc.pubmedtypeJournal Article
dc.rights.accessRightsopen access
dc.subjectAlternative Splicing
dc.subjectCtIP
dc.subjectDNA Damage Response
dc.subjectSF3B
dc.titleCtIP -mediated alternative mRNA splicing finetunes the DNA damage response.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number27
dspace.entity.typePublication

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