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Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

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dc.contributor.authorMontserrat-de la Paz, Sergio
dc.contributor.authorRodriguez-Martin, Noelia M.
dc.contributor.authorVillanueva, Alvaro
dc.contributor.authorPedroche, Justo
dc.contributor.authorCruz-Chamorro, Ivan
dc.contributor.authorMillan, Francisco
dc.contributor.authorMillan-Linares, Maria C.
dc.contributor.authoraffiliation[Montserrat-de la Paz,S; Cruz-Chamorro,I] Department of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Seville, Spain. [Rodriguez-Martin,NM; Villanueva,A; Pedroche,J; Millan,F; Millan-Linares,MC] Department of Food & Health, Instituto de la Grasa, CSIC, Seville, Spain. [Cruz-Chamorro,I] Instituto de Biomedicina de Sevilla, IBiS (Universidad de Sevilla, HUVR, Junta de Andalucía, CSIC), Seville, Spain. [Millan-Linares,MC] Cell Biology Unit, Instituto de la Grasa, CSIC, Seville, Spain.
dc.contributor.funderGrant CYTED-2019 119RT0567 supported this work from the Spanish Ministry of Science, Innovation, and Universities.
dc.date.accessioned2022-09-20T09:30:40Z
dc.date.available2022-09-20T09:30:40Z
dc.date.issued2020-06-30
dc.description.abstractBioactive protein hydrolysates have been identified in several sources as possible agents in the prevention and treatment of many diseases. A wheat gluten (WG) concentrate was hydrolyzed by Alcalase under specific conditions. The resulting hydrolysates were evaluated by in vitro cell-free experiments leading to the identification of one bioactive WG protein hydrolysate (WGPH), which was used at 50 and 100 μg/mL on primary human monocytes. Reactive oxygen species (ROS) and nitrite levels and RT-qPCR and ELISA techniques were used to analyze the functional activity of WGPH. Our results showed that WGPH hydrolyzed in 45 min (WGPH45A) down-regulated gene expression of Interleukin (IL)-1β, IL-6, IL-17, and Interferon gamma (IFNγ) and reduced cytokine release in lipopolysaccharide (LPS)-stimulated monocytes. In addition, WGPH45A down-regulated gene-related to atherosclerotic onset. Our results suggest that WGPH45A has a potent anti-inflammatory and atheroprotective properties, reducing the expression of gene-related inflammation and atherosclerosis that could be instrumental in maintaining cardiovascular homeostasis.es_ES
dc.description.versionYeses_ES
dc.identifier.citationMontserrat-de la Paz S, Rodriguez-Martin NM, Villanueva A, Pedroche J, Cruz-Chamorro I, Millan F, et al. Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes. Foods. 2020 Jun 30;9(7):854es_ES
dc.identifier.doi10.3390/foods9070854es_ES
dc.identifier.essn2304-8158
dc.identifier.pmcPMC7404777
dc.identifier.pmid32630013es_ES
dc.identifier.urihttp://hdl.handle.net/10668/4076
dc.journal.titleFoods
dc.language.isoen
dc.page.number16 p.
dc.publisherMDPIes_ES
dc.relation.publisherversionhttps://www.mdpi.com/2304-8158/9/7/854/htmes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectWheat glutenes_ES
dc.subjectProtein hydrolysateses_ES
dc.subjectAtherosclerosises_ES
dc.subjectMonocyteses_ES
dc.subjectCeliac diseasees_ES
dc.subjectHidrolisados de proteínaes_ES
dc.subjectAterosclerosises_ES
dc.subjectMonocitoses_ES
dc.subjectEnfermedad celíacaes_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Interferons::Interferon-gammaes_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycoconjugates::Lipopolysaccharideses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Protein Hydrolysateses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Oxygen Compounds::Reactive Oxygen Specieses_ES
dc.subject.meshMedical Subject Headings::Anatomy::Hemic and Immune Systems::Immune System::Leukocytes::Leukocytes, Mononuclear::Monocyteses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-6es_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Poaceae::Triticumes_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-17es_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Electrolytes::Ions::Anions::Nitriteses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Subtilisinses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutenses_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Peripheral Nervous System Agents::Sensory System Agents::Analgesics::Analgesics, Non-Narcotic::Anti-Inflammatory Agents, Non-Steroidales_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expressiones_ES
dc.subject.meshMedical Subject Headings::Diseases::Cardiovascular Diseases::Vascular Diseases::Arterial Occlusive Diseases::Arteriosclerosis::Atherosclerosises_ES
dc.subject.meshMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammationes_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasises_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Molecular Probe Techniques::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assayes_ES
dc.titleEvaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocyteses_ES
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication

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