Publication:
Cytotoxicity, Epidermal Barrier Function and Cytokine Evaluation after Antiseptic Treatment in Bioengineered Autologous Skin Substitute.

dc.contributor.authorGarcia-Valdivia, Marta
dc.contributor.authorQuiñones-Vico, Maria I
dc.contributor.authorOrtega-Llamas, Laura
dc.contributor.authorFernandez-Gonzalez, Ana
dc.contributor.authorUbago-Rodriguez, Ana
dc.contributor.authorSanabria-de la Torre, Raquel
dc.contributor.authorArias-Santiago, Salvador
dc.contributor.funderMinistry of Health and Families of the Andalusian Regional Government
dc.contributor.funderCarlos III Health Institute
dc.contributor.funderMinistry of Science, Innovation and Universities of Spain
dc.date.accessioned2023-05-03T13:48:36Z
dc.date.available2023-05-03T13:48:36Z
dc.date.issued2022-06-17
dc.description.abstractBioengineered autologous skin substitutes (BASS) technology is an emerging field for skin burn therapy. However, further studies on BASS characterization, viability against standard procedures for wound healing, and protocol optimization are necessary for the improvement of BASS technology for clinical use. The aim of this study is to evaluate the effect of common antiseptics for clinical use in BASS, focusing on cell viability, inflammatory cytokine pattern, and epithelium and skin barrier integrity, in order to establish the most adequate treatment for wound care after BASS grafting. Human keratinocytes (hKT) and dermal fibroblasts (hDF) were isolated from foreskin samples and integrated into hyaluronic acid-based BASS. The following antiseptics were applied every 48 h: ethanol (70%), chlorhexidine digluconate (1%), sodium hypochlorite (0.02%), povidone iodine (100 mg/mL), and polyhexanide (0.1%), during a follow-up of 16 days. Sodium hypochlorite was the only treatment that showed a high cell viability percentage throughout the evaluation time compared to other antiseptic treatments, as well as a similar cytokine secretion pattern as control BASS. No significant differences were found regarding epidermal barrier function. These findings point towards sodium hypochlorite being the least aggressive antiseptic treatment for BASS post-transplantation wound care.
dc.description.sponsorshipWe gratefully acknowledge financial support from the Ministry of Health and Families of the Andalusian Regional Government (PIGE-0242-2019) and from the Carlos III Health Institute (PI17/02083). The work of María I. Quiñones Vico was supported by a predoctoral fellowship (BOE22/10/2019) from the Ministry of Science, Innovation and Universities of Spain.
dc.description.versionSi
dc.identifier.doi10.3390/biomedicines10061453
dc.identifier.issn2227-9059
dc.identifier.pmcPMC9220084
dc.identifier.pmid35740473
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220084/pdf
dc.identifier.unpaywallURLhttps://www.mdpi.com/2227-9059/10/6/1453/pdf?version=1655963821
dc.identifier.urihttp://hdl.handle.net/10668/20837
dc.issue.number6
dc.journal.titleBiomedicines
dc.journal.titleabbreviationBiomedicines
dc.language.isoen
dc.organizationHospital Universitario Virgen de las Nieves
dc.organizationInstituto de Investigación Biosanitaria de Granada (ibs.GRANADA)
dc.organizationFundación Pública Andaluz Progreso y Salud-FPS
dc.publisherMDPI AG
dc.pubmedtypeJournal Article
dc.relation.projectIDPIGE-0242-2019
dc.relation.projectIDPI17/02083
dc.relation.projectIDBOE22/10/2019
dc.relation.publisherversionhttps://www.mdpi.com/resolver?pii=biomedicines10061453
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectbioengineered autologous skin substitute
dc.subjectcell viability
dc.subjectcytokine secretion
dc.subjectdrug development
dc.subjectepidermal barrier function
dc.subjectin vitro model
dc.subjectregenerative medicine
dc.subjecttissue engineering
dc.subject.decsClorhexidina
dc.subject.decsHipoclorito de Sodio
dc.subject.decsPovidona Yodada
dc.subject.decsSupervivencia celular
dc.subject.decsÁcido Hialurónico
dc.subject.meshPovidone-Iodine
dc.subject.meshchlorhexidine gluconate
dc.subject.meshSodium Hypochlorite
dc.subject.meshHyaluronic Acid
dc.subject.meshpolihexanide
dc.subject.meshCell Survival
dc.titleCytotoxicity, Epidermal Barrier Function and Cytokine Evaluation after Antiseptic Treatment in Bioengineered Autologous Skin Substitute.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication

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