Publication:
The neuroprotective transcription factor ATF5 is decreased and sequestered into polyglutamine inclusions in Huntington's disease.

dc.contributor.authorHernández, Ivó H
dc.contributor.authorTorres-Peraza, Jesús
dc.contributor.authorSantos-Galindo, María
dc.contributor.authorRamos-Morón, Eloísa
dc.contributor.authorFernández-Fernández, M Rosario
dc.contributor.authorPérez-Álvarez, María J
dc.contributor.authorMiranda-Vizuete, Antonio
dc.contributor.authorLucas, José J
dc.date.accessioned2023-01-25T09:51:33Z
dc.date.available2023-01-25T09:51:33Z
dc.date.issued2017-08-31
dc.description.abstractActivating transcription factor-5 (ATF5) is a stress-response transcription factor induced upon different cell stressors like fasting, amino-acid limitation, cadmium or arsenite. ATF5 is also induced, and promotes transcription of anti-apoptotic target genes like MCL1, during the unfolded protein response (UPR) triggered by endoplasmic reticulum stress. In the brain, high ATF5 levels are found in gliomas and also in neural progenitor cells, which need to decrease their ATF5 levels for differentiation into mature neurons or glia. This initially led to believe that ATF5 is not expressed in adult neurons. More recently, we reported basal neuronal ATF5 expression in adult mouse brain and its neuroprotective induction during UPR in a mouse model of status epilepticus. Here we aimed to explore whether ATF5 is also expressed by neurons in human brain both in basal conditions and in Huntington's disease (HD), where UPR has been described to be partially impaired due to defective ATF6 processing. Apart from confirming that ATF5 is present in human adult neurons, here we report accumulation of ATF5 within the characteristic polyglutamine-containing neuronal nuclear inclusions in brains of HD patients and mice. This correlates with decreased levels of soluble ATF5 and of its antiapoptotic target MCL1. We then confirmed the deleterious effect of ATF5 deficiency in a Caenorhabditis elegans model of polyglutamine-induced toxicity. Finally, ATF5 overexpression attenuated polyglutamine-induced apoptosis in a cell model of HD. These results reflect that decreased ATF5 in HD-probably secondary to sequestration into inclusions-renders neurons more vulnerable to mutant huntingtin-induced apoptosis and that ATF5-increasing interventions might have therapeutic potential for HD.
dc.identifier.doi10.1007/s00401-017-1770-2
dc.identifier.essn1432-0533
dc.identifier.pmid28861715
dc.identifier.unpaywallURLhttps://digital.csic.es/bitstream/10261/214094/1/LucasJJ_NeuroprotectionTranscription.pdf
dc.identifier.urihttp://hdl.handle.net/10668/11549
dc.issue.number6
dc.journal.titleActa neuropathologica
dc.journal.titleabbreviationActa Neuropathol
dc.language.isoen
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.organizationHospital Universitario Virgen del Rocío
dc.page.number839-850
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rights.accessRightsopen access
dc.subjectATF5
dc.subjectER stress
dc.subjectHuntington’s disease
dc.subjectMCL1
dc.subjectNeuroprotection
dc.subjectUPR
dc.subject.meshActivating Transcription Factors
dc.subject.meshAnimals
dc.subject.meshApoptosis
dc.subject.meshCaenorhabditis elegans
dc.subject.meshCaenorhabditis elegans Proteins
dc.subject.meshCell Line, Tumor
dc.subject.meshDisease Models, Animal
dc.subject.meshEndoplasmic Reticulum Stress
dc.subject.meshHumans
dc.subject.meshHuntingtin Protein
dc.subject.meshHuntington Disease
dc.subject.meshInclusion Bodies
dc.subject.meshMice, Transgenic
dc.subject.meshNeurons
dc.subject.meshNeuroprotection
dc.subject.meshPeptides
dc.titleThe neuroprotective transcription factor ATF5 is decreased and sequestered into polyglutamine inclusions in Huntington's disease.
dc.typeresearch article
dc.type.hasVersionSMUR
dc.volume.number134
dspace.entity.typePublication

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