Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii.

dc.contributor.authorCayô, Rodrigo
dc.contributor.authorRodríguez, María-Cruz
dc.contributor.authorEspinal, Paula
dc.contributor.authorFernández-Cuenca, Felipe
dc.contributor.authorOcampo-Sosa, Alain A
dc.contributor.authorPascual, Alvaro
dc.contributor.authorAyala, Juan A
dc.contributor.authorVila, Jordi
dc.contributor.authorMartínez-Martínez, Luis
dc.contributor.authoraffiliation[Cayô,R; Rodríguez,MC; Ocampo-Sosa,AA; Martínez-Martínez,L] Service of Microbiology, University Hospital Marqués de Valdecilla—IFIMAV [Espinal,P; Vila,J] Service of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona. [Fernández-Cuenca,F; Pascual,A] Service of Microbiology, University Hospital Virgen Macarena, Seville. [Ayala,JA] Centro de Biología Molecular Severo Ochoa—CSIC-UAM, Campus de Cantoblanco, Madrid, Spain. [Rodríguez,MC; Martínez-Martínez,L] Department of Molecular Biology, School of Medicine, University of Cantabria,
dc.contributor.funderThis work was partially supported by the Instituto de Salud Carlos III, Spanish Network for Research in Infectious Diseases (REIPI, RD06/0008), and FIS (PI080209). We are grateful to the Coordenacao de Aperfeicoamento de Pessoal de Nível Superior (CAPES), which gave a PDEE grant to R.C. (protocol 4149/08-4). We acknowledge the funding of MICINN (BFU2009-09200) to J.A.A. L. Dijkshoorn is thanked for providing A. baumannii strains RUH-134 and RUH-875.
dc.descriptionJournal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractThere is limited information on the role of penicillin-binding proteins (PBPs) in the resistance of Acinetobacter baumannii to β-lactams. This study presents an analysis of the allelic variations of PBP genes in A. baumannii isolates. Twenty-six A. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in Spain were included. The antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. Based on the six complete genomes of A. baumannii, the PBP genes were identified, and primers were designed for each gene. The nucleotide sequences of the genes identified that encode PBPs and the corresponding amino acid sequences were compared with those of ATCC 17978. Seven PBP genes and one monofunctional transglycosylase (MGT) gene were identified in the six genomes, encoding (i) four high-molecular-mass proteins (two of class A, PBP1a [ponA] and PBP1b [mrcB], and two of class B, PBP2 [pbpA or mrdA] and PBP3 [ftsI]), (ii) three low-molecular-mass proteins (two of type 5, PBP5/6 [dacC] and PBP6b [dacD], and one of type 7 (PBP7/8 [pbpG]), and (iii) a monofunctional enzyme (MtgA [mtgA]). Hot spot mutation regions were observed, although most of the allelic changes found translated into silent mutations. The amino acid consensus sequences corresponding to the PBP genes in the genomes and the clinical isolates were highly conserved. The changes found in amino acid sequences were associated with concrete clonal patterns but were not directly related to susceptibility or resistance to β-lactams. An insertion sequence disrupting the gene encoding PBP6b was identified in an endemic carbapenem-resistant clone in one of the participant
dc.identifier.citationCayô R, Rodríguez MC, Espinal P, Fernández-Cuenca F, Ocampo-Sosa AA, Pascual A, et al. Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii. Antimicrob. Agents Chemother. 2011; 55(12):5907-13es
dc.journal.titleAntimicrobial agents and chemotherapy
dc.publisherAmerican Society for Microbiologyes
dc.rights.accessRightsopen access
dc.subjectSecuencia de Aminoácidoses
dc.subjectHospitales Escuelaes
dc.subjectPruebas de Sensibilidad Microbianaes
dc.subjectProteínas de Unión a las Penicilinases
dc.subjectAnálisis de Secuencia de ADNes
dc.subjectResistencia beta-Lactámicaes
dc.subject.meshMedical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Moraxellaceae::Acinetobacter::Acinetobacter baumanniies
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Amino Acid Sequencees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agentses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Carbapenemses
dc.subject.meshMedical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Academic Medical Centers::Hospitals, Teachinges
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Testses
dc.subject.meshMedical Subject Headings::Information Science::Information Science::Information Services::Documentation::Molecular Sequence Dataes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Penicillin-Binding Proteinses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation::Point Mutationes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNAes
dc.subject.meshMedical Subject Headings::Geographicals::Geographic Locations::Europe::Spaines
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial::beta-Lactam Resistancees
dc.subject.meshMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Moraxellaceae Infections::Acinetobacter Infectionses
dc.titleAnalysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter
dc.typeresearch article
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