Publication:
Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells.

dc.contributor.authorDíaz-Tocados, Juan M
dc.contributor.authorHerencia, Carmen
dc.contributor.authorMartínez-Moreno, Julio M
dc.contributor.authorMontes de Oca, Addy
dc.contributor.authorRodríguez-Ortiz, Maria E
dc.contributor.authorVergara, Noemi
dc.contributor.authorBlanco, Alfonso
dc.contributor.authorSteppan, Sonja
dc.contributor.authorAlmadén, Yolanda
dc.contributor.authorRodríguez, Mariano
dc.contributor.authorMuñoz-Castañeda, Juan R
dc.date.accessioned2023-01-25T09:50:30Z
dc.date.available2023-01-25T09:50:30Z
dc.date.issued2017-08-10
dc.description.abstractMesenchymal stem cells (MSC) are osteoblasts progenitors and a variety of studies suggest that they may play an important role for the health in the field of bone regeneration. Magnesium supplementation is gaining importance as adjuvant treatment to improve osteogenesis, although the mechanisms involving this process are not well understood. The objective of this study was to investigate the effects of magnesium on MSC differentiation. Here we show that in rat bone marrow MSC, magnesium chloride increases MSC proliferation in a dose-dependent manner promoting osteogenic differentiation and mineralization. These effects are reduced by 2-APB administration, an inhibitor of magnesium channel TRPM7. Of note, magnesium supplementation did not increase the canonical Wnt/β-catenin pathway, although it promoted the activation of Notch1 signaling, which was also decreased by addition of 2-APB. Electron microscopy showed higher proliferation, organization and maturation of osteoblasts in bone decellularized scaffolds after magnesium addition. In summary, our results demonstrate that magnesium chloride enhances MSC proliferation by Notch1 signaling activation and induces osteogenic differentiation, shedding light on the understanding of the role of magnesium during bone regeneration.
dc.identifier.doi10.1038/s41598-017-08379-y
dc.identifier.essn2045-2322
dc.identifier.pmcPMC5552799
dc.identifier.pmid28798480
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552799/pdf
dc.identifier.unpaywallURLhttps://www.nature.com/articles/s41598-017-08379-y.pdf
dc.identifier.urihttp://hdl.handle.net/10668/11496
dc.issue.number1
dc.journal.titleScientific reports
dc.journal.titleabbreviationSci Rep
dc.language.isoen
dc.organizationHospital Universitario Reina Sofía
dc.organizationHospital Universitario Reina Sofía
dc.organizationInstituto Maimónides de Investigación Biomédica de Córdoba-IMIBIC
dc.page.number7839
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAnimals
dc.subject.meshBone and Bones
dc.subject.meshBoron Compounds
dc.subject.meshCell Differentiation
dc.subject.meshCell Proliferation
dc.subject.meshCells, Cultured
dc.subject.meshEnzyme Inhibitors
dc.subject.meshMagnesium Chloride
dc.subject.meshMesenchymal Stem Cells
dc.subject.meshMicroscopy, Electron
dc.subject.meshOsteogenesis
dc.subject.meshRats
dc.subject.meshReceptors, Notch
dc.subject.meshSignal Transduction
dc.subject.meshTRPM Cation Channels
dc.titleMagnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number7
dspace.entity.typePublication
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