Publication:
Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

Loading...
Thumbnail Image

Date

2020-12-22

Authors

Soriano, José L.
Calpena, Ana C.
Rodríguez-Lagunas, María J.
Domènech, Òscar
Bozal-de Febrer, Nuria
Garduño-Ramírez, María L.
Clares, Beatriz

Advisors

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI
Metrics
Google Scholar
Export

Research Projects

Organizational Units

Journal Issue

Abstract

The main goal of this work is the study of the skin wound healing efficacy of an antioxidant cocktail consisting of vitamins A, D, E and the endogenous pineal hormone melatonin (MLT), with all of these loaded into a thermosensitive hydrogel delivery system. The resulting formulation was characterized by scanning electron microscopy. The antioxidant efficacy and microbiological activity against Gram positive and Gram negative strains were also assayed. The skin healing efficacy was tested using an in vivo model which included histological evaluation. Furthermore, atomic force microscopy was employed to evaluate the wound healing efficacy of rat skin burns through the determination of its elasticity at the nanoscale using force spectroscopy analysis. The resulting hydrogel exhibited sol state at low temperature and turned into a gel at 30 ± 0.2 °C. The hydrogel containing the antioxidant cocktail showed higher scavenging activity than the hydrogel containing vitamins or MLT, separately. The formulation showed optimal antimicrobial activity. It was comparable to a commercial reference. It was also evidenced that the hydrogel containing the antioxidant cocktail exhibited the strongest healing process in the skin burns of rats, similar to the assayed commercial reference containing silver sulfadiazine. Histological studies confirmed the observed results. Finally, atomic force microscopy demonstrated a similar distribution of Young's modulus values between burned skin treated with the commercial reference and burned skin treated with hydrogel containing the antioxidant cocktail, and all these with healthy skin. The use of an antioxidant cocktail of vitamins and MLT might be a promising treatment for skin wounds for future clinical studies.

Description

MeSH Terms

Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Melatonin
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Sulfonamides::Sulfanilamides::Sulfadiazine::Silver Sulfadiazine
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Electron::Microscopy, Electron, Scanning
Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Motivation::Goals
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Scanning Probe::Microscopy, Atomic Force
Medical Subject Headings::Phenomena and Processes::Biological Phenomena::Biological Processes::Regeneration::Wound Healing
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Spectrum Analysis
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Growth Substances::Micronutrients::Vitamins

DeCS Terms

CIE Terms

Keywords

Vitamins, Melatonin, Antioxidant, Skin, Healing, ATF microscopy, Vitaminas, Melatonina, Antioxidantes, Piel, Cicatrización de heridas, Microscopía de fuerza atómica

Citation

Soriano JL, Calpena AC, Rodríguez-Lagunas MJ, Domènech Ò, Bozal-de Febrer N, Garduño-Ramírez ML, et al. Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns. Pharmaceutics. 2020 Dec 22;13(1):8