Publication:
Role of APOBEC3H in the Viral Control of HIV Elite Controller Patients.

dc.contributor.authorBenito, Jose M
dc.contributor.authorHillung, Julia
dc.contributor.authorRestrepo, Clara
dc.contributor.authorCuevas, Jose M
dc.contributor.authorLeon, Agathe
dc.contributor.authorRuiz-Mateos, Ezequiel
dc.contributor.authorPalacios-Muñoz, Rosario
dc.contributor.authorGorgolas, Miguel
dc.contributor.authorSanjuan, Rafael
dc.contributor.authorRallon, Norma
dc.date.accessioned2023-01-25T10:02:42Z
dc.date.available2023-01-25T10:02:42Z
dc.date.issued2017-10-12
dc.description.abstractBackground APOBEC3H (A3H) gene presents variation at 2 positions (rs139297 and rs79323350) leading to a non-functional protein. So far, there is no information on the role played by A3H in spontaneous control of HIV. The aim of this study was to evaluate the A3H polymorphisms distribution in a well-characterized group of Elite Controller (EC) subjects. Methods We analyzed the genotype distribution of two different SNPs (rs139297 and rs79323350) of A3H in 30 EC patients and compared with 11 non-controller (NC) HIV patients. Genotyping was performed by PCR, cloning and Sanger sequencing. Both polymorphisms were analyzed jointly in order to adequately attribute the active or inactive status of A3H protein. Results EC subjects included in this study were able to maintain a long-term sustained spontaneous HIV-viral control and optimal CD4-T-cell counts; however, haplotypes leading to an active protein were very poorly represented in these patients. We found that the majority of EC subjects (23/30; 77%) presented allelic combinations leading to an inactive A3H protein, a frequency slightly lower than that observed for NC studied patients (10/11; 91%). Conclusions The high prevalence of non-functional protein coding-genotypes in EC subjects seems to indicate that other innate restriction factors different from APOBEC3H could be implicated in the replication control exhibited by these subjects.
dc.description.sponsorshipWe want to particularly acknowledge the patients in this study for their participation and to the HIV BioBank integrated in the Spanish AIDS Research Network (RIS) and collaborating Centers for the generous gifts of clinical samples used in this work. The HIV BioBank, integrated in the Spanish AIDS Int. J. Med. Sci. 2018, Vol. 15 http://www.medsci.org 100 Research Network, is supported by Institute of Health Carlos III, ISCIII, Spanish Health Ministry (Grant nº RD06/0006/0035 and RD12/0017/0037) as part of the State Plan for Scientific and Technical Research and Innovation and cofinanced by ISCIII – Sub-Directorate General for Research Assessment and Promotion and European Regional Development Fund (ERDF) and Foundation for Research and Prevention of AIDS in Spain (FIPSE). This study would not have been possible without the collaboration of medical, nursery staff and data managers who have taken part in the project (Supplementary Text S1). The RIS Cohort (CoRIS) is funded by the ISCIII through the Spanish AIDS Research Network (RIS C03/173 and RD12/0017/0018) as part of the State Plan for Scientific and Technical Research and Innovation and cofinanced by ISCIII – Sub-Directorate General for Research Assessment and Promotion and European Regional Development Fund (ERDF).
dc.description.sponsorshipFunding This work has been (partially) funded by the RD12/0017/0031, RD12/0017/0033, RD16/0025/0013 and CP14/00198 projects as part of the Health Research and Development Strategy, State Plan for Scientific and Technical Research and Innovation (2008-2011; 2013-2016) and cofinanced by Institute of Health Carlos III, ISCIII – Sub-Directorate General for Research Assessment and Promotion and European Regional Development Fund (ERDF). Norma Rallón is a Miguel Servet investigator from the ISCIII (CP14/00198), Madrid, Spain. Julia Hillung was funded by project RD12/0017/0033, and Clara Restrepo was funded by project D12/0017/0031 and is currently funded by project RD16/0025/0013.
dc.description.version
dc.identifier.citationBenito JM, Hillung J, Restrepo C, Cuevas JM, León A, Ruiz-Mateos E, et al. Role of APOBEC3H in the viral control of HIV elite controller patients. Int J Med Sci. 2018 Jan 1;15(2):95-100
dc.identifier.doi10.7150/ijms.22317
dc.identifier.essn1449-1907
dc.identifier.pmcPMC5765721
dc.identifier.pmid29333092
dc.identifier.pubmedURLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765721/pdf
dc.identifier.unpaywallURLhttps://www.medsci.org/v15p0095.pdf
dc.identifier.urihttp://hdl.handle.net/10668/12011
dc.issue.number2
dc.journal.titleInternational journal of medical sciences
dc.journal.titleabbreviationInt J Med Sci
dc.language.isoen
dc.organizationHospital Universitario Virgen de la Victoria
dc.organizationInstituto de Investigación Biomédica de Málaga-IBIMA
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.page.number6
dc.provenanceRealizada la curación de contenido 20/09/2024
dc.publisherBlue Haven NSW: Ivyspring International
dc.pubmedtypeJournal Article
dc.pubmedtypeMulticenter Study
dc.relation.publisherversionhttps://doi.org/10.7150/ijms.22317
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAPOBEC3H polymorphisms
dc.subjectHIV
dc.subjectelite controllers.
dc.subjectrs139297
dc.subjectrs79323350
dc.subject.decsAdult
dc.subject.decsAminohydrolases
dc.subject.decsCD4-Positive T-Lymphocytes
dc.subject.decsCross-Sectional Studies
dc.subject.decsFemale
dc.subject.meshAdult
dc.subject.meshAminohydrolases
dc.subject.meshCD4-Positive T-Lymphocytes
dc.subject.meshCross-Sectional Studies
dc.subject.meshFemale
dc.subject.meshGene Frequency
dc.subject.meshHIV Infections
dc.subject.meshHaplotypes
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshPolymorphism, Single Nucleotide
dc.subject.meshVirus Replication
dc.titleRole of APOBEC3H in the Viral Control of HIV Elite Controller Patients.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number15
dspace.entity.typePublication

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